Mary Schad and Lucas Berliew
Physical and Biological Sciences Category
“Exploring the potential of Flavopereirine for breast cancer at the molecular level”
Mary Schad, Lucas Berliew,, and Narinder K. Sharma
Department of Biology and Environmental Health, Missouri Southern State University, MO
Breast cancer is one of the deadliest forms of cancer and is the second most common cause of death for women after lung cancer. Many potential drugs do not work well due to the resistance and complexity of the molecular pathways that trigger the growth and division of breast cancer cells, highlighting the need for continuous urgent exploration of drugs and molecular targets in breast cancer. The objective of the present study is to determine the potential use of Flavopereirine in controlling breast cancer. Breast cancer cells were exposed to the varying concentration of drug along with negative control to analyze the effect of the drug on cancer cell viability. We have found a 5 micromolar concentration of Flavopereirine to inhibit about 50% cell viability using XTT assay. Thereafter, cells were grown with drug and whole cells were harvested to analyze the effect of the drug on gene expression. RNA from the drug-treated and control cells was isolated, followed by cDNA synthesis. We have performed quantitative real-time PCR to analyze the expression of key genes involved in metastasis. Our preliminary results indicate that this drug does not impact the expression of two metastasis-related genes i.e. E-Cadherin and N-Cadherin.