Lucas Berliew and Maya Courtade
Biology Major
Physical and Biological Sciences Category
Bio
Maya Courtade and Lucas Berliew
Department of Biology and Environmental Health, Missouri Southern State University, MO
Abstract
Prostate cancer is the most common type of non-skin cancer found in and it is the second leading cause of cancer death in men in Western societies. PI3K (phosphoinositide 3-kinase)/mTOR (mammalian target of rapamycin)/AKT pathway is one of the main molecular pathways that is abruptly active in prostate cancer. In the present study, we have analyzed the effect of BEZ235 on the growth of prostate cancer cell line LNCaP by using XTT assay. We have found that 1 micromolar concentration of BEZ 235 was quite potent on LNCaP cells. We have further treated the cells with BEZ235 and isolated RNA for quantitative gene expression analysis. BEZ 235 enhances the apoptosis of LNCaP cells as analyzed by apoptosis markers. We are further analyzing the expression of the genes involved in PI3 kinase pathways impacted by BEZ235.